The ABI PRISM® 3100-Avant Genetic Analyzer is an advanced capillary electrophoresis system designed for a wide range of genetic analysis applications. It is particularly well-suited for low-to-medium throughput laboratories that require a fully automated solution for sequencing and fragment analysis. Here’s a detailed look at the 3100-Avant Genetic Analyzer and its controller:

 

The ABI PRISM® 3100-Avant Genetic Analyzer Features

Automated Capillary Electrophoresis

The 3100-Avant Genetic Analyzer uses capillary electrophoresis to separate, detect, and analyze fluorescent-labeled DNA fragments. This process is highly automated, allowing for unattended operation and the analysis of hundreds of samples in a single day.

Upgradeable System

One of the key features of the 3100-Avant is its upgradeability. Initially equipped with four capillaries, it offers a pathway to expand to 16 capillaries, quadrupling the daily throughput without the need for an additional instrument.

Ultra Rapid Sequencing Module

The system includes an Ultra Rapid Sequencing module that increases throughput by more than 30%, providing high-quality sequencing reads in excess of 500 base pairs with 98.5% accuracy. This module enhances the system’s productivity and ease of use for various applications, including resequencing, SNP discovery, and validation, as well as microsatellite analysis.

Multiple Dye Detection and Supported Applications

The 3100-Avant supports multiple dye sets and applications, ensuring flexibility for different research needs. It can perform sequencing analysis, fragment sizing, validation, and screening with ease.

Software and Algorithms

The system comes with a computer workstation for instrument control and data analysis. It includes Sequencing Analysis Software for basecalling and GeneScan® Analysis Software for fragment analysis. These tools provide easy instrument setup and automated polymer filling and sample injection from both 96- and 384-well microtiter plate formats.

Capillary Arrays and Separation Matrix

The internally uncoated capillaries come in pre-assembled arrays of four, available in various lengths to support multiple applications and run methodologies. The capillary arrays are designed for use with industry-standard 96- and 384-well microtiter plates and are specified to last for 150 runs.

Reagents and Polymers

The 3100-Avant system can use either POP-4™ or POP-6™ polymers as the separation matrix. Applied Biosystems provides a range of reagents for sequencing and fragment analysis, including BigDye® Terminator kits and the SNaPshot® Multiplex Kit.

Safety and Documentation

Thermo Fisher Scientific provides comprehensive safety information and technical support for the 3100-Avant Genetic Analyzer. Users have access to material safety data sheets (MSDSs), waste disposal guides, and technical support through various channels.

The ABI PRISM® 3100-Avant Genetic Analyzer is a sophisticated instrument that offers automation, flexibility, and high throughput for genetic analysis. Its upgradeable nature, combined with the Ultra Rapid Sequencing module and support for multiple applications, makes it a valuable asset for any research lab focused on genetic analysis. The system’s ease of use, coupled with robust software and reliable reagents, ensures that it delivers consistent and high-quality results for a variety of genetic research applications.

 

 

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