The ABI PRISM® 310 Genetic Analyzer is a highly versatile and automated single-capillary genetic analyzer developed by Applied Biosystems, now part of Thermo Fisher Scientific. It is designed to perform a wide range of sequencing and fragment analysis applications, making it a valuable tool in research, clinical, forensic labs, and educational settings. Here’s an in-depth look at the 310 Genetic Analyzer:
ABI PRISM® 310 Genetic Analyzer Features
Automation and Ease of Use
The 310 Genetic Analyzer integrates ABI PRISM® multicolor fluorescent labeling, capillary electrophoresis (CE), and software for data analysis to achieve unparalleled ease of use. The system automates virtually all aspects of analysis, from sample and gel loading to data collection and analysis, which significantly streamlines laboratory workflows.
Flexible System for Various Applications
This genetic analyzer is flexible enough to handle a variety of applications, including comparative sequencing, linkage analysis, short tandem repeat (STR) analysis, single nucleotide polymorphism (SNP) detection, discovery and validation, mutation detection, and more. Its adaptability makes it suitable for a broad spectrum of genetic research.
Capillary Electrophoresis Technology
The 310 system employs capillary electrophoresis, a method that separates DNA fragments by size and charge through a capillary filled with a polymer. This technology allows for high-resolution applications such as DNA sequencing and rapid runs for applications like microsatellite analysis.
Performance Optimized Polymers (POP)
The system uses Performance Optimized Polymers (POPTM) for DNA separation, which yields extremely reproducible results. The POP-6TM polymer is used for high-resolution applications, while the POP-4TM polymer provides faster runs for other applications.
Automated Sample Loading
The autosampler of the 310 Genetic Analyzer uses an electro-kinetic injection to sequentially load each sample into the capillary for electrophoresis. This feature contributes to the system’s high throughput and consistent performance.
Specifications and Throughput
The 310 Genetic Analyzer boasts impressive specifications, including a basecalling accuracy of 98.5% and the ability to make thousands of base calls per day. It can sequence up to 600 bases with the standard DNA sequencing protocol and 400 bases with the rapid DNA sequencing protocol.
User-Friendly Software
The system is equipped with user-friendly software that simplifies the operation for users of all experience levels. The software supports customization of run naming, sequence prioritization, and data export options, enhancing the flexibility and usability of the system.
Safety and Support
Applied Biosystems provides comprehensive safety information and technical support for the 310 Genetic Analyzer. Users have access to material safety data sheets (MSDSs), waste disposal guides, and technical support through various channels.
The ABI PRISM® 310 Genetic Analyzer stands out for its automation, flexibility, and ease of use. It is a powerful instrument that has revolutionized genetic analysis by providing accurate, reproducible, and rapid results. Its wide range of applications and user-friendly features make it an essential tool for genetic analysis in various fields. Whether for de novo sequencing, SNP validation, or linkage mapping, the 310 Genetic Analyzer delivers reliable performance that researchers and technicians can count on.
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